[Retracted] Adipose-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicles Rescue Tendon Injury in Rat via the miR-19 a/IGFBP3 Axis

Figure 4

Effects of miR-19a on oxidative stress and proliferation of tenocytes. (a–d) qRT-PCR was used to detect the expression of miR-19a in EVs. (a, b) Detect EVs obtained by high-speed differential centrifugation. (c, d) Detect EVs obtained by Exosome Purification Kit. (e) qRT-PCR was used to detect the expression of miR-19a in exosomes secreted from tendon tissues. (f–i) ELISA was used to detect the levels of CK, LDH, MDA, and SOD both in the NC mimic group, miR-19a mimic group, ASO-NC group, and ASO-miR-19a group. (j) MTT was used to detect the activity of tenocytes. (k) FCM was performed to detect the apoptosis of tenocytes. (l) The expression of apoptosis-related proteins was detected by Western blot in the NC mimic group, miR-19a mimic group, ASO-NC group, and ASO-miR-19a group (, , and ).

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