Cyclin-Dependent Kinase 1 Inhibition Potentiates the Proliferation of Tonsil-Derived Mesenchymal Stem Cells by Delaying Cellular Senescence

Figure 1

Changes in senescent markers with serial passaging of TMSCs. (a) Morphological changes of young and old TMSC groups were determined by measuring cell length and width. Young TMSCs exhibited a polygonal morphology, whereas old TMSCs formed dispersed shapes. (b) Senescent cells were identified as blue-stained cells under an optical microscope. Magnification: ×200; scale bar, 50 μm. The orange arrowhead indicates stained cells. Senescent cells were identified as blue-stained cells and were calculated by the average absorbance intensity of SA-β-gal staining (405 nm) from 5 randomly selected fields between young and old TMSCs. (c) Gene expression of hTERT and TRF-1 which are telomere length markers in TMSCs. (d) Cell proliferation profile of young and old TMSCs during 64 h. The blue triangle line indicates young TMSCs, and the orange rectangle line indicates old TMSCs. (e, f) The TMSCs from each experimental group were stained with PI staining solution and analyzed their cell cycle stage. The values were considered statistically significant at and .

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