An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool

Figure 1

Validation of CASH-1–specific gRNAs. (a) Schematic representation of designed gRNAs and their target. (b) The T7E1 endonuclease assay after transfection of a relevant gRNA into HEK293T cells. The ImageJ software was employed for the calculation of indel percentages. L: 100-bp ladder.

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