Interleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells

Figure 1

Intracellular localization of IL21R in ADSCs and induced to adipogenesis. Immunofluorescence labeling of undifferentiated ADSCs using α-IL21R, α-ATP5B, and α-ALDH4A1 in a confocal microscope (a, b). ADSCs were incubated with the primary antibody α-IL21R and the secondary antibody conjugated to Alexa488. ADSCs were incubated with the primary antibody α-ATP5B (Atp5b ATP synthase located in the mitochondria) or ALDH4A1 and the secondary antibody conjugated to Alexa 546. ADSCs were incubated with DAPI for nuclear staining. (a, b) Images were then merged and showed that α-IL21R is colocalized with α-ATP5B and ALDH4A1, indicating that the protein is located in the mitochondria and within cell nuclei. (c, d) Localization of IL21R in premature adipocyte (c) and in adipocyte containing more lipid vesicles (d). Scale bar: 50 μm. Representative images of the donor TAL36 (a–d).

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