Single-Cell Transcriptome Analysis Defines Expression of Kabuki Syndrome-Associated KMT2D Targets and Interacting Partners

Figure 4

Chromatin structures of Kmt2d, Rbbp5, Axin2, and Kdm4b in the mouse incisor dental pulp. (a) ATAC-seq analysis indicated that the chromatin is accessible in Kmt2d. Strong peaks were detected in the transcription start site (TSS) marked with a red arrow. hmeDIP-seq results showed 5hmC enrichment in gene bodies (compare to the input), which correlates well with RNA expression. The brown arrow indicates the direction of transcription. (b) The hmeDIP-seq and ATAC-seq peaks are enriched in the promoter (blue arrow) and TSS (red arrow) of Rbbp5. The brown arrow indicates the direction of Rbbp5 expression. (c) The ATAC-seq and hmeDIP-seq peaks are enriched in the gene body of Axin2. A strong ATAC-seq signal was also detected in the TSS (red arrow). The direction of Axin2 expression is marked with a brown arrow. (d) A strong ATAC-seq peak is present in the TSS (red arrow) of Kdm4b. 5hmC is enriched within the gene body of Kdm4b. The brown arrow indicates the direction of Kdm4b expression.

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