Knockdown of Adra2a Increases Secretion of Growth Factors and Wound Healing Ability in Diabetic Adipose-Derived Stem Cells

Figure 4

Clonidine reduces the expression and secretion of growth factors in C57BL/6 ASCs. The mRNA expression levels of VEGF (a), HGF (b), and TGF-β (c) at 0 h, 4 h, 6 h, 12 h, and 24 h of C57BL/6 ASCs treated with 10 μM and 20 μM of clonidine, respectively. Secretion of VEGF (d), HGF (e), and TGF-β (f) at 6 h, 24 h, 48 h, and 72 h of C57BL/6 ASCs treated with 10 μM clonidine. Clonidine reduces contents of cAMP and PKA in C57BL/6 ASCs. The contents of cAMP (g) and PKA (h) at 0 min, 5 min, 15 min, and 30 min with 20 μM clonidine treatment. Three biological replicates and three technical replicates were used in each case. ; .

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