Knockdown of Adra2a Increases Secretion of Growth Factors and Wound Healing Ability in Diabetic Adipose-Derived Stem Cells

Figure 5

Adra2a knocking down increases the expression and secretion of growth factors in T2D ASCs. Representative micrographs of T2D ASCs and T2D ASCs with lentivirus. Green represents lentivirus successfully transfected into the cells (a, b). The levels of expression of Adra2a from T2D ASCs transfected with Adra2a knockdown lentivirus and negative control lentivirus (c). The expression of VEGF (d), HGF (e), and TGF-β (f) in the NC, NC+C, knockdown, and knockdown+C group at 24 h, 48 h, and 72 h, respectively. The secretion of VEGF (g), HGF (h), and TGF-β (i) in the NC, NC+C, knockdown, and knockdown+C group at 24 h, 48 h, and 72 h, respectively. The change of cAMP and PKA in T2D ASCs after Adra2a knocking down (j, k): the contents of cAMP (j) and activities of PKA (k) at 0 min, 5 min, and 15 min with 10 μM clonidine treatment. Note: NC: Negative control, T2D ASCs transfected with control vectors; knockdown: Adra2a knocked-down T2D ASCs; NC+C: Negative control T2D ASCs treated with 10 μM clonidine; knockdown+C: Adra2a knocked-down T2D ASCs treated with 10 μM clonidine. Three biological replicates and three technical replicates were used in each case. ; .

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