Low-Intensity Pulsed Ultrasound Enhanced Adipose-Derived Stem Cell-Mediated Angiogenesis in the Treatment of Diabetic Erectile Dysfunction through the Piezo-ERK-VEGF Axis

Figure 7

LIPUS activated the ERK-VEGFA pathway in ADSCs. (a) Western blot analysis of ERK, p-ERK, VEGFA, and GAPDH expression in ADSCs treated without or 0 minutes, 10 minutes, 30 minutes, 60 minutes, and 120 minutes after LIPUS stimulation. (b) The relative density of p-ERK compared with that of ERK. (c) The relative density of VEGF compared with that of GAPDH. (d) Western blot analysis of ERK, p-ERK, VEGFA, and GAPDH expression in ADSCs treated with LIPUS or PD98059, an ERK1/2 pathway inhibitor (ADSCs were pretreated with 80 μM PD98059 for 1 hour). (e) The relative density of p-ERK compared with that of ERK. (f) The relative density of VEGF compared with that of GAPDH. (g) The concentration of VEGF in the supernatant of the cell culture medium. Data are presented as the mean ± standard deviation. ns when comparing the PD98059 group. when comparing the NC group.

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