Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine <i>OCT4</i> Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells

<div>Schematic overview of the study on the porcine <i>OCT4</i> reporter system during early embryo development and embryonic stem cells. Porcine embryos were microinjected with the p<i>OCT4</i>-<i>Δ</i>PE-eGFP (DE-GFP) containing a distal enhancer and core promoter and p<i>OCT4</i>-<i>Δ</i>DE-DsRed2 (PE-RFP) containing a proximal enhancer and core promoter. Hatched parthenogenetic blastocysts were seeded on feeder cells composed of mitotically inactivated mouse embryonic fibroblasts (MEFs). Protein and mRNA expression in morula, blastocysts, embryonic stem cells, and embryoid body was analyzed using immunocytochemistry and quantitative real-time PCR.</div>

Stem Cells International

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Figure 1: Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine <i>OCT4</i> Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells 

Figure 1 | Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine OCT4 Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells 