Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine OCT4 Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells

Figure 2

Expression of the OCT4 reporter system during preimplantation porcine embryo development. (a) Expression levels of DE-GFP and PE-GFP were measured in 3 developmental stages (6–8 cells, early blastocyst and late blastocyst). Error bars represent the mean SEM. Values with different letters (a, b, and c) are significantly different. (b) Gene expression patterns of PE-GFP and DE-GFP in the OCT4 reporter system-injected embryos. Each group has three replicates. Significant differences. (, ). (c–e) Immunofluorescence analysis for PE-RFP, DE-GFP, pluripotent genes (OCT4 and SOX2–yellow), and DAPI nuclear staining in OCT4 reporter system-injected embryos. Size maker corresponds to 100 μm.

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