Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine OCT4 Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells

Figure 4

Expression of dual-porcine OCT4 reporter system and pluripotency markers in porcine embryonic stem cells.Reporter fluorescent genes and pluripotency markers were checked in the protein expression level. (a) Porcine embryonic stem cells with the OCT4 reporter system. (b) Porcine embryonic stem cells without the OCT4 reporter system. (c) Porcine embryonic fibroblasts with the OCT4 reporter system. (d) Embryonic bodies after differentiation from porcine embryonic stem cells with the OCT4 reporter system. (e) Porcine embryonic stem cells without the reporter system. (f) Porcine embryonic stem cells with the reporter system. Flow cytometry showed that porcine embryonic stem cells with the OCT4 reporter system have 99.6% positive rates of fluorescence compared to those of the control group.

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