Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine OCT4 Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells

Figure 5

Comparison of the distal/proximal enhancer activity rate in pluripotent cells of mouse or pig. The activity of OCT4 distal and proximal enhancers was compared in various pluripotent cells. Cells with a naïve pluripotent state (mouse E14 ESCs, porcine embryo) had a value greater than 1, which indicates higher activity of the distal enhancer than the proximal enhancer. On the other hand, cells known as the primed pluripotent state (mouse P19 ECs, FGF- or LIF-dependent piPSCs) had a value less than 1, which indicated that the proximal enhancer has higher activity than the distal enhancer. E14 mouse embryonic stem cells, P19 mouse embryonic carcinoma, porcine embryo, FGF- or LIF-dependent porcine induced pluripotent stem cells, and porcine embryonic stem cells with FGF- or LIF-supplemented culture media. Mouse E14 and P19 cells were analyzed by using luciferase assay in (Kim et al., 2019), and porcine-induced pluripotent stem cells were analyzed in (Kim et al., 2021).