Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells

Figure 1

The quantity of seeding cells affected the process of hiPSCs differentiated into macrophages. (a) Schematic diagram of the protocols of macrophage differentiation from hiPSCs. (b) Representative bright-field images of EBs and cells at different time points during culture stages under two macrophage differentiation protocols. Scale bars, 250 μm. (c) The percentages of different cell types (CD34⁺, CD45⁺, CD45⁺CD14⁺, and CD45⁺CD11b⁺) produced during the different culture stages under two macrophage differentiation protocols. (d) The percentage of CD34⁺CD45⁺ cells at day 14 and CD45⁺CD14⁺/CD45⁺CD11b⁺ cells at day 14, day 22, and day 30 in two macrophage differentiation protocols. and . (e) The total number of cells at day 14, day 22, and day 30 in two macrophage differentiation protocols. . (f) Representative Wright-Giemsa staining images of monocytes and macrophages in peripheral blood and cells differentiated from hiPSCs at day 14, day 22, and day 30 in the P3500 protocol. Scale bar represents 50 μm.

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