Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells

<div>More seeding cells promoted the development of HPCs in EBs. (a–c) Frozen sections and immunofluorescence images of EBs in two macrophage differentiation protocols. Immunocytochemical analysis of CD34 (red) and CD45 (green) at day 4, day 6, and day 12. DAPI (blue) shows the cell nuclei (scale bars: 25 <i>μ</i>m in (a) and (b), 50 <i>μ</i>m in (c)).</div>

Stem Cells International

fig2

Figure 2

Figure 2: Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells 