CXCR4⁺ Sorted Adipose-Derived Stem Cells Enhance Their Functional Benefits and Improve Cardiac Function after Myocardial Infarction

Figure 2

CXCR4⁺ ASCs were isolated and sorted by fluorescence-activated cell sorting (FACS). (a) Only a minor population of CXCR4⁺ ASCs (≈2%) was detected in unfractionated ASCs. After sorting, CXCR4⁺ ASCs were enriched to approximately 63%. (b) CXCR4 (red) was highly expressed in CXCR4⁺ sorted ASCs by immunofluorescence staining. (c) The mRNA expression of CXCR4 was significantly augmented in CXCR4⁺ sorted ASCs relative to unfractionated ASCs by quantitative PCR. (d) CXCR4 protein (39 kDa) was more strongly expressed in CXCR4⁺ sorted ASCs than in unfractionated ASCs. Scale bar represents 50 μm. versus unfractionated ASCs. Abbreviations: CXCR4: CXC-chemokine receptor 4; ASCs: adipose-derived stem cells; FACS: fluorescence-activated cell sorting; GAPDH: glyceraldehyde-3-phosphate dehydrogenase, DAPI, RNA, deoxyribonucleic acid; DAPI; 4,6-diamidino-2-phenylindole.

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