CXCR4⁺ Sorted Adipose-Derived Stem Cells Enhance Their Functional Benefits and Improve Cardiac Function after Myocardial Infarction

Figure 3

CXCR4⁺ sorted ASCs exhibited the better viability, proliferation, cytokine secretion, and migration than did unfractionated ASCs in vitro. (a) CXCR4⁺ sorted ASCs had markedly increased cell viability and proliferation rates compared with unfractionated ASCs. (b) CXCR4⁺ sorted ASCs secreted a higher level of angiogenic growth factors including VGEF, HGF, and IGF-1 relative to unfractionated ASCs. (c) Images were taken at 0 hour and 24 hours after scratch to visualize migrated cells and wound healing. (d) Scratch assay found a significantly higher wound closure percentage in CXCR4⁺ sorted ASCs than in unfractionated ASCs. Scale bar represents 200 μm. versus unfractionated ASCs. Abbreviations: CXCR4: CXC-chemokine receptor 4; ASCs: adipose-derived stem cells; VEGF: vascular endothelial growth factor; HGF: hepatocyte growth factor; IGF-1: insulin-like growth factor.

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