Bone Marrow Culture-Derived Conditioned Medium Recovers Endothelial Function of Vascular Grafts following In Vitro Ischemia/Reperfusion Injury in Diabetic Rats

Figure 3

Effect of ischemia/reperfusion (IR) injury on aortic caspase-3, caspase-8, caspase-9, and caspase-12 expression in control and diabetic rats. Semiquantitative scoring of (a) caspase-3 (control, rats; control-IR, rats; DM, rats; DM-IR, rats), (b) caspase-8 (control, rats; control-IR, rats; DM, rats; DM-IR, rats), (c) caspase-9 (control, rats; control-IR, rats; DM, rats; DM-IR, rats), and (d) caspase-12 (control, rats; control-IR, rats; DM, rats; DM-IR, rats) immunohistochemical staining of thoracic aortic rings obtained from nondiabetic and diabetic rats. DM indicates diabetes mellitus. The evaluation was conducted by two examiners blinded to the experimental groups from 4 randomized nonoverlapping fields per aorta per rat. Data are represented as the and correspond to the mean of all pictures. versus control, ^# versus control-IR, and ^$ versus DM.

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