Bone Marrow Culture-Derived Conditioned Medium Recovers Endothelial Function of Vascular Grafts following In Vitro Ischemia/Reperfusion Injury in Diabetic Rats

Figure 5

Effects of bone marrow culture-derived conditioned medium (CM) against ischemia/reperfusion (IR) injury on contraction and relaxation in the aorta of nondiabetic control and diabetic rats. Contractile responses for (a, b) phenylephrine (expressed as the percentage of 80 mM potassium chloride (KCl)-induced contraction) and (c, d) high potassium K⁺-induced depolarization (control, rings from 8 rats; control-IR, rings from 8 rats; control-IR+CM, rings from 10 rats; DM, rings from 8 rats; DM-IR, rings from 10 rats; DM-IR+CM, rings from 11 rats), and (e, f) acetylcholine-induced endothelium-dependent vasorelaxation (control, rings from 8 rats; control-IR, rings from 8 rats; control-IR+CM, rings from 10 rats; DM, rings from 8 rats; DM-IR, rings from 11 rats; DM-IR+CM, rings from 11 rats) and (g, h) sodium nitroprusside-induced endothelium-independent vasorelaxation (control, rings from 8 rats; control-IR, rings from 8 rats; control-IR+CM, rings from 10 rats; DM, rings from 8 rats; DM-IR, rings from 11 rats; DM-IR+CM, rings from 11 rats) of isolated thoracic aortic rings obtained from control and diabetic rats. DM indicates diabetes mellitus. Data are represented as the . versus corresponding control or DM; ^# versus corresponding control-IR or DM-IR.

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