Bone Marrow Culture-Derived Conditioned Medium Recovers Endothelial Function of Vascular Grafts following In Vitro Ischemia/Reperfusion Injury in Diabetic Rats

Figure 6

Effects of bone marrow culture-derived conditioned medium (CM) against ischemia/reperfusion (IR) injury on caspase-3 (control, rats; control-IR, rats; control-IR+CM, rats; DM, rats; DM-IR, rats; DM-IR+CM, rats), caspase-8 (control, rats; control-IR, rats; control-IR+CM, rats; DM, rats; DM-IR, rats; DM-IR+CM, rats), caspase-9 (control, rats; control-IR, rats; control-IR+CM, rats; DM, rats; DM-IR, rats; DM-IR+CM, rats), and caspase-12 (control, rats; control-IR, rats; control-IR+CM, rats; DM, rats; DM-IR, rats; DM-IR+CM, rats) expression. Semiquantitative scoring of (a, b) caspase-3, (c, d) caspase-8, (e, f) caspase-9, and (g, h) caspase-12 immunohistochemical staining in the aortic rings obtained from nondiabetic control and diabetic rats. DM indicates diabetes mellitus. The evaluation was conducted by two examiners blinded to the experimental groups from 4 randomized non-overlapping fields per aorta per rat. Data are represented as the and correspond to the mean of all pictures. versus corresponding control or DM; ^# versus corresponding control-IR or DM-IR.

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