Bone Marrow Culture-Derived Conditioned Medium Recovers Endothelial Function of Vascular Grafts following In Vitro Ischemia/Reperfusion Injury in Diabetic Rats

Figure 7

Effects of bone marrow culture-derived conditioned medium (CM) against ischemia/reperfusion (IR) injury on aortic DNA strand breaks. Representative merged micrographs of aortic tissue shows pink nuclei with fragmented DNA, visualized by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining and blue nuclei represents 4,6-diamino-2-phenylindole staining (magnification ×40; scale bar: 100 μm) followed by quantification of TUNEL-positive cells in the aortic rings obtained from (a) nondiabetic control and (b) diabetic rats. DM indicates diabetes mellitus. The evaluation was conducted by two examiners blinded to the experimental groups from 4 randomized non-overlapping fields per aorta per rat. Data are represented as the . versus corresponding control or DM, ^# versus corresponding control-IR or DM-IR. Control, rats; control-IR, rats; control-IR+CM, rats; DM, rats; DM-IR, rats; DM-IR+CM, rats.

(a)

(b)