The Combined Use of Platelet-Rich Plasma Clot Releasate and Allogeneic Human Umbilical Cord Mesenchymal Stem Cells Rescue Glucocorticoid-Induced Osteonecrosis of the Femoral Head

Figure 4

Effects of PRCR, UC-MSCs, and PRCR + UC-MSCs on DEX-treated BMSCs and HUVEC apoptosis. (a, b) Representative images of TUNEL-positive cells (apoptotic cells) of DEX-treated BMSCs and HUVEC apoptosis in different coculture conditions. (c) Quantitative analysis of apoptosis rates of BMSCs and HUVECs, respectively. The PRCR and PRCR+UC-MSCs rescued the DEX-induced apoptotic effects (). (d) Western blotting was conducted to evaluate the expression of the apoptosis-related proteins. The PRCR, UC-MSC, and PRCR + UC-MSC groups significantly blocked caspase-3 activation and increased Bcl-2 expression compared to the DEX group (, ^#Comparisons between the DEX group and other groups, ; Comparisons between the two groups, ). PRCR: platelet-rich plasma clot releasate; UC-MSCs: umbilical cord mesenchymal stem cells; DEX: dexamethasone; HBMSCs: human bone marrow mesenchymal stem cells; HUVECs: human umbilical vein endothelial cells; TUNEL: terminal transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labeling; DAPI: 4, 6-diamidino-2-phenylindole; GADPH: glyceraldehyde-3-phosphate dehydrogenase.

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