YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma

Figure 4

Inhibition of the YTHDC1 expression significantly limits tumor cell stemness maintenance, migration, and proliferation. (a) Western blotting of YTHDC1 in six HNSCC cell lines. (b) Western blotting of YTHDC1 in SCC9 and HN4 with or without YTHDC1 knockdown. (c) qPCR data of YTHDC1 in SCC9 and HN4 with or without YTHDC1 knockdown. (d) SOX2 and BMI1 protein expression in SCC9 and HN4 with or without YTHDC1 knockdown. (e) The OCT4 and NANOG protein expression in SCC9 and HN4 with or without YTHDC1 knockdown. (f). SCC9 and HN4 cell proliferation ability was suppressed after the YTHDC1 expression was decreased. (g) Sphere formation ability of SCC9 and HN4 cell lines was inhibited after the YTHDC1 expression was suppressed. (h) Representative images and quantification of migration assay of SCC9 and HN4 cell lines with or without YTHDC1 knockdown. (i) Representative images and quantification of colony formation assay of SCC9 and HN4 cell lines with or without YTHDC1 knockdown. (j). Tumor xenograft experiment in nude mice detects the effect of YTHDC1 on tumor growth in vivo ( in each group). (k) Tumor growth curve. The quantitative value of tumor volumes is plotted, and tumor volumes are measured once two days after injected. (l) Scatter diagram shows tumor weight.

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