Mesenchymal Stem Cells Ameliorate Hyperglycemia in Type I Diabetic Developing Male Rats

Figure 6

Electron micrographs of a pancreatic acinar cell of control developing rat demonstrating (a) zymogen (Z) granules, arrays of rough endoplasmic reticulum (RER), and mitochondria (M) with basally located nucleus (N); (b) parts of β, α, and δ cells; (c) parts of two β cells with spherical-shaped nuclei (N). β granules (g) have dark central core surrounded by an electron lucent halo (arrows). Electron micrographs of pancreas acinar cells of diabetic developing rat showing (d) decrease of the zymogen (Z) granules; (e) shrinkage and pyknotic nucleus (N), vacuolized mitochondria (M), and extensive fragmented arrays of rough endoplasmic reticulum (RER); (f) β cell in Langerhans islet of the pancreas displaying cytoplasmic vacuolation (V), obvious decrease of secretory granules (arrow), and pyknotic nucleus (N). Electron micrograph of pancreatic acinar cells of a diabetic developing rat treated with mesenchymal stem cells during early period (diabetic+MSCs1 group) illustrating (g) the acinar cells appeared nearly similar to the normal form with basally located normal nuclei (N) and normal aggregation of zymogen (Z) granules with few degenerated mitochondria (M) and arrays of rough endoplasmic reticulum (RER). (h) Aggregation of β cells which appeared nearly like the control group, euchromatic oval nucleus (N); few vacuoles in β cells and increase of the secretory granules (arrows) surrounded by electron lucent halo; (i) oval nucleus (N); increase in the secretory granules (arrows) with some vacuolized mitochondria (M). Few vacuoles are also seen (V). Electron micrographs of (j, k) pancreatic acinar cells and (l) β cell in Langerhans islet of a diabetic developing rat treated with mesenchymal stem cells in a late period (diabetic+MSCs2 group) showing (a) hemorrhage edema (HE) among the acini with congestion in the blood vessels (). (b) Damaged mitochondria (M); dilated rough endoplasmic reticulum (RER), zymogenic granules (Z), and an irregular nuclear envelope (arrowheads). (c) Vacuolized mitochondria (M) lost most their cristae and a scarcity of β granules in some β cells (). Fusion of some granules (F) is also seen. The nucleus (N) of islet β cell appeared with an irregular shape.

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