DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status

Figure 3

Pathway alteration after DPSC supernatant treatment. (a) Bubble diagram of the pathways enriched in the KEGG pathway, Reactome, and PANTHER databases. (b) KEGG classification of the DEGs after DPSC treatment. (c) Circos map of enriched KEGG pathways. (d) The interactive bar charts display the biological pathways that are overrepresented in the upregulated and downregulated genes generated by Enrichr. (e) Western blot results and gray value analysis results showing the pathway-related proteins in NPs after specific treatments. “TNF-α” represents NP cells cultured in 500 ng/ml TNF-α. “DPSC” represents NP cells cultured in culture media containing DPSC culture supernatant (1 : 3 v/v). After 2 days of treatment, cell lysates were analyzed by western blotting. Gray values were analyzed by ImageJ. “Relative expression” represents the gray value of a specific protein divided by that of GAPDH. and . KEGG: Kyoto Encyclopedia of Genes and Genomes.

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