Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhance Alveolar Type II Cell Proliferation and Attenuate Lung Inflammation in a Rat Model of Bronchopulmonary Dysplasia

Figure 2

hUCMSC treatment attenuates lung morphology and function in the IA-LPS BPD model. (a) The study design represents the IA-LPS BPD model and hUCMSC treatment. Transtracheal administration of hUCMSCs was conducted on PN7. Study end points on PN14 involved analysis of lung morphology, function, and right ventricular hypertrophy (RVH). (b) Representative lung sections stained with H&E on PN14,  μm. (c) Quantification of MLI (, ANOVA, ). (d) Quantification of radial alveolar counts (RAC) (, ANOVA, ). (e) Comparison of pups’ weight among four groups (, ANOVA). (f) Representative immunofluorescence images of vWF staining in the lung on PN14 in each group,  μm. (g) Quantification of vWF-positive vessels (<100 μm) (, ANOVA, and ). (h) Lung resistance (LR) and (i) dynamic compliance (Cdyn) were determined from anesthetized and ventilated pups (, ANOVA, ). (j) The right ventricular index was determined by to measure RVH (, ANOVA, ).

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