Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhance Alveolar Type II Cell Proliferation and Attenuate Lung Inflammation in a Rat Model of Bronchopulmonary Dysplasia

Figure 9

MVs activate the PTEN/p-AKT pathway and suppress the MAPK pathway in the IA-LPS BPD model. (a) The protein levels of PTEN, p-AKT at S473, and AKT were evaluated by western blotting on lung homogenates on PN14. (b) Densitometric analysis was used to quantify the expression of PTEN and p-AKT/AKT (, ANOVA, and ). (c) The protein levels of p38, JNK, ERK, and their phosphorylated forms (p-p38, p-JNK, and p-ERK) were evaluated by western blotting on lung homogenates on PN14. (d) Densitometric analysis was used to quantify the phosphorylation of p38 at Thr180/Tyr182, JNK at Thr183/Tyr185, and ERK1/2 at Thr202/Tyr1204 (, ANOVA, and ). (e) The protein levels of VEGF-A in lung homogenate were evaluated by western blotting on PN14. (f) Densitometric analysis was used to quantify the expression of VEGF-A (, ANOVA, NS: not significant, ).

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