MAPK/ERK-CBP-RFPL-3 Mediates Adipose-Derived Stem Cell-Induced Tumor Growth in Breast Cancer Cells by Activating Telomerase Reverse Transcriptase Expression

Figure 5

ASC induction of CBP upregulation enhances telomerase activity by activating the binding of RFPL-3 to the hTERT promoter region in breast cancer cells. (a) Chromatin immunoprecipitation assays were performed using anti-RFPL3 in normal breast cells and breast cancer cells. The amplified products of the hTERT promoter were analyzed by agarose gel electrophoresis. IgG was used as a negative control. (b) Chromatin immunoprecipitation analysis of the proteins bound to the hTERT promoter was performed using anti-CBP in breast cancer cells, and then, the pulled-down protein complex was examined by immunoblotting using anti-CBP. (c) The immunoprecipitation assay of nuclear extracts from breast cancer cells was carried out using anti-RFPL3, and then, the complexes were analyzed by immunoblotting using anti-CBP. (d–e) Chromatin immunoprecipitation analysis of the proteins bound to the hTERT promoter was performed using anti-RFPL3 and anti-CBP in breast cancer cells cocultured with ASCs. (f) The telomerase activity in MDA-MB-231 cells transfected with CBP vectors was measured using a TeloTAGGG telomerase PCR ELISAs. .

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