Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response

Figure 1

Secretion and knockdown efficiency of the TSG-6. (a) TSG-6 levels in supernatants of control, sham serum, and burn serum groups were evaluated by ELISA. Compared with control and sham groups, the TSG-6 levels in the burn serum group were significantly increased. (b) Representative photographs demonstrated successful transfection with Lenti-GFP-siRNA (vehicle) and Lenti-TSG-6-siRNA (siTSG-6) in hUC-MSCs (the inverted fluorescence microscope, 100x). (c) The levels of TSG-6 protein expression in hUC-MSCs after transfection with Lenti-GFP-siRNA and Lenti-TSG-6-siRNA were detected by Western blotting, and quantitative analysis is shown in the corresponding histogram. (d, e) TSG-6 levels in lung tissue and serum of burn and burn+hUC-MSC groups were also evaluated using ELISA. TSG-6 level of the burn+hUC-MSC group was significantly higher than that of the burn group. Values are represented as (). Asterisk () and double asterisk () stand for and , respectively.

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