Long Noncoding RNA IGFBP7-AS1 Promotes Odontogenesis of Stem Cells from Human Exfoliated Deciduous Teeth via the p38 MAPK Pathway

Figure 2

lncRNA and mRNA profiles of stem cells from human exfoliated deciduous teeth (SHED) during odontogenic differentiation. (a) Volcano plot showing 569 lncRNAs upregulated and 569 lncRNAs significantly downregulated on day 7, and 767 lncRNAs upregulated and 591 lncRNAs significantly downregulated on day 14 ( or ≤-1.3, ). The Venn diagram shows differentially expressed lncRNAs during odontogenic differentiation of SHED. (b) Volcano plot showing 343 mRNAs upregulated and 219 mRNAs downregulated on day 7, and 437 mRNAs upregulated and 323 mRNAs downregulated on day 14 ( or ≤-1.3, ) on gene level. The Venn diagram shows differentially expressed mRNAs during odontogenic differentiation of SHED. (c) Gene Ontology (GO) analysis of mRNAs differentially expressed during odontogenic differentiation in SHED on day 7. (d) GO analysis of mRNAs differentially expressed during odontogenic differentiation in SHED on day 14. (e) qRT-RCR validation of selected lncRNAs differentially expressed during odontogenic differentiation: ENST00000333145, ENST00000508328, ENST00000524152, and ENST00000590622. (f) Results of RNA-sequencing data for selected lncRNAs. Data were expressed as the (SD) of three experiments per group, , , , and .

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