Long Noncoding RNA IGFBP7-AS1 Promotes Odontogenesis of Stem Cells from Human Exfoliated Deciduous Teeth via the p38 MAPK Pathway

Figure 4

IGFBP7-AS1 overexpression increases the odontogenic differentiation of SHED. (a) Fluorescence microscope and qRT-PCR detected the transfection effect of IGFBP7-AS1 overexpression lentiviruses in the NC and IGFBP7-AS1 groups. . (b) Images of ALP staining of SHED on day 7 after odontogenic differentiation and ARS staining of SHED on day 14 after odontogenic differentiation in the NC and IGFBP7-AS1 groups. (c) qRT-PCR detected osteo/odontogenic differentiation-related markers: ALP, DSPP, DMP1, OCN, and BMP2 in the NC and IGFBP7-AS1 groups. (d) Western blot detected odontogenic differentiation-specific markers: DSPP and DMP1 in the NC and IGFBP7-AS1 groups. Data were expressed as the (SD) of three experiments per group, , , and .

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