The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level

Figure 1

PSCs Characterization. (a) Schematic representation of study design. (b c) All emerged colonies (TSA-iPSCs, TSA+ iPSCs, IVF-ESCs, and TSA+ NT-ESCs) represented typical ESC-like morphology. (d) ALP activity in 100 nM TSA-treated iPSCs (a), 50 nM TSA-treated iPScs (b), TSA-negative iPSCs (c), and ImageJ analysis showed 50 nM and 100 nM TSA concentration significantly increases the ALP expression in generated iPSCs (g). TSA treated ESCs (d), nontreated ESCs (e), and IVF-ESCs (f) all showed high ALP activity (h). (e) Normal karyotype of cell lines was confirmed by karyotyping. (f) The established PSCs express typical pluripotency markers, including OCT4 (green) and SSEA-1 (red). Nuclei were stained with DAPI (blue). (g, h) Fluorescent intensity of OCT4 and SSEA1 in iPSC and ESC colonies was quantified by ImageJ. indicates the statistical significance () compared to the control group. represents .# indicates the statistical significance () between groups.

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