High-Fat Diet Increases Bone Loss by Inducing Ferroptosis in Osteoblasts

Figure 2

Chemical staining of mouse femurs with hematoxylin and eosin (H&E) after 12 weeks of feeding mice on normal and high-fat diets revealed a significant reduction in the number of metaphyseal trabeculae in the femur, which was then restored to some extent after administration of ferroptosis inhibitors (a); scale bar represents 200 μm. Experiments revealed that after mice consumed a diet high in fat content, the number of alkaline phosphatase- (ALP-) positive osteoblasts on the surface of the trabeculae in the femur was significantly reduced (b), and the reduction in the number of osteoblasts was somewhat mitigated by a high-fat diet accompanied by treatment with ferroptosis inhibitors in mice. Osteoblasts are shown in brown and nuclei in green. Quantification of the number of osteoblasts per bone surface (b). Scale bars represent 100 μm, , , . Quantification of staining for TRAP+ cells in the femoral bone marrow cavity (a, c), with osteoclasts in red and nuclei in green (a) and NS indicating no statistical difference in component; scale bars represent 100 μm, .

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