High-Fat Diet Increases Bone Loss by Inducing Ferroptosis in Osteoblasts

Figure 3

To demonstrate whether ferroptosis occurred in bone tissue of mice fed a high-fat diet, the expression of Gpx4 protein, a key protein for ferroptosis, in the femur of mice was examined using immunofluorescence staining. A significant decrease in the number of Gpx4 positive cells in the bone marrow of mice fed a high-fat diet was found (a), and after administration of a ferroptosis inhibitor, the number of Gpx4-positive cells increased significantly compared to the group fed a high-fat diet alone (a, b). Gpx4-positive cells in red and DAPI-stained nuclei in blue. , , . By measuring biochemical indicators of ferroptosis in the normal diet group, high-fat diet group, and high-fat diet plus ferroptosis inhibitor group, iron content (c), the total GSH content (d), GSH/GSSG ratio (e), and MDA content (f) in the femurs of mice were found to be , , ; NS indicates no statistical difference.

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