High-Fat Diet Increases Bone Loss by Inducing Ferroptosis in Osteoblasts

Figure 5

To verify that osteogenic formation is affected to some extent in a high-fat environment. We treated Mc3t3-E1 cells in high-fat medium and then replaced the osteogenic induction medium for seven days of osteogenic differentiation culture. We found that the level of alkaline phosphatase ALP was significantly reduced in high-fat conditioned medium-treated cells at day seven of osteogenic induction culture (a, b) and that this process could be alleviated by ferroptosis inhibitors. , , . Early intracellular osteogenic differentiation was measured by immunofluorescence at day seven of osteogenic differentiation in high-fat-treated cells Osterix (c), Rux2 (d), and Ocn (e) found to be reduced to varying degrees. The addition of Fer-1, a ferroptosis inhibitor, to the high-fat medium treatment significantly reduced the expression of osteogenic marker proteins (c–e).

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