Small Extracellular Vesicles from Inflamed Adipose Derived Stromal Cells Enhance the NF-κB-Dependent Inflammatory/Catabolic Environment of Osteoarthritis

Figure 1

sEVs_IL-1 Surface Epitope characterization. (a) Whole MACSPlex Exosome Kit surface assessment of 37 surface markers and 2 isotype controls of different purified sEVs_IL-1 preparations () from IL-1β treated ADSC (black graphs). Data are expressed as net fluorescence intensity (control subtracted fluorescence intensity) and displayed as . (b) Side-by-side comparison of the expression of tetraspanins (CD9, CD81, and CD63) in sEVs obtained from IL-1β treated ADSC (sEVs_IL-1, , black graphs) or untreated ADSC (sEVs, , white graphs). (c) Side-by-side comparison of the expression of other 11 detectable surface markers in sEVs obtained from IL-1β treated ADSC (sEVs_IL-1, , black graphs) or untreated ADSC (sEVs, , white graphs). The latter correspond to data presented in [5]. Since the ADSC cultures were the same, comparisons were undertaken by mean of the Student’s t-test for paired samples and statistically significant differences evidenced as and .

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