Small Extracellular Vesicles from Inflamed Adipose Derived Stromal Cells Enhance the NF-κB-Dependent Inflammatory/Catabolic Environment of Osteoarthritis

Figure 8

sEVs_IL-1 effects on NF-κB activation. (a) p65 immunofluorescence on chondrocytes (upper row) and synoviocytes (lower row) in control conditions (CTR) and at 4 and 15 h after exposure to sEVs_IL-1. Scale bar: 10 μm. Each image reports the level of the colocalization of the red (p65) to the blue (DNA) signal. The colocalization of the fluorochromes (shown in white) was quantified using Pearson’s colocalization coefficient (), derived from 15 analyzed optical sections and expressed as . 5 cells were considered for each condition. (b) Western blot analysis of samples of chondrocytes (left) and synoviocytes (right) in CTR or sEVs_IL-1 condition at both 4 hr and 15 hr post-delivery. These samples were dedicated to western blot analysis of phosphorylation of p65 and β-actin as loading control. Lower graphs: relative quantification of phospho-p65 signal normalized to that of β-actin.

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