Small Extracellular Vesicles from Inflamed Adipose Derived Stromal Cells Enhance the NF-κB-Dependent Inflammatory/Catabolic Environment of Osteoarthritis

Table 1

List of primers used in real-time PCR.

RNA template	Primer sequences; (forward:5 and reverse:3)	Annealing temperature (°C)
GAPDH	5-TGGTATCGTGGAAGGACTCATGAC; 3-ATGCCAGTGAGCTTCCCGTTCAGC	60
IL-1β	5-GTGGCAATGAGGATGACTTGTT; 3-TGGTGGTCGGAGATTCGTAG	60
IL-6	5-TAGTGAGGAACAAGCCAGAG; 3-GCGCAGAATGAGATGAGTTG	60
IL-8	5-CCAAACCTTTCCACCC; 3-ACTTCTCCACAACCCT	60
MCP-1	5-GAAGCTCGCACTCTCGCCT; 3-GAGTGTTCAAGTCTTCGGA	60
TNF-α	5-AGCCCATGTTGTAGCAAACC; 3-ACCTGGGAGTAGATGAGGTA	60
MMP-1	5-TGGACCTGGAGGAAATCTTG; 3-CCGCAACACGATGTAAGTTG	60
MMP-10	5-GCCAGTCCATGGAGCAAGGCT; 3-TCGCCTAGCAATGTAACCAGCTGT	60
ADAMTS4	5-CTGCCTACAACCACCG; 3-GCAACCAGAACCGTCC	60
ADAMTS5	5-GCACTTCAGCCACCATCAC; 3-AGGCGAGCACAGACATCC	60
MMP-13	5-TCACGATGGCATTGCT; 3-GCCGGTGTAGGTGTAGA	60
COX-2	5-CAGCACTTCACGCATCAGTTT; 3-GCGCAGTTTACGCTGTCTA	60
INOS	5-ACATTGATCAGAAGCTGTCCCAC; 3-AAAGGCTGTGAGTCCTGCAC	60
IL-10	5-CTTTAAGGGTTACCTGGGTTG; 5-CTTGATGTCTGGGTCTTGG	60
VEGF	5-TGATGATTCTGCCCTCCTC; 3-GCCTTGCCTTGCTGCTC	60