Research Article
[Retracted] RNA-Sequence Reveals the Regulatory Mechanism of miR-149 on Osteoblast Skeleton under Mechanical Tension
Figure 5
Regulation of cytoskeleton and osteogenesis of MG63 cells by ASAP3. (a) RT-qPCR detected the mRNA expression of ASAP3. (b, c) Western blot detected the levels of ASAP3 protein. (d, e) F-actin fluorescence intensity was detected by rhodamine-labeled phalloidin staining. Compared with the si-NC group, , . (f) Western blot detected the protein levels of ASAP3 (g), F-actin (h), BMP2 (i), RUNX2 (j), AKT (k), and p-AKT (l). Compared with the pcDNA-NC group, , , and ; ns: no significant difference.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |
| (f) |
| (g) |
| (h) |
| (i) |
| (j) |
| (k) |
| (l) |