[Retracted] lncRNA HOTTIP Recruits EZH2 to Inhibit PTEN Expression and Participates in IM Resistance in Chronic Myeloid Leukemia

Figure 2

(a) IR-K562 was transfected with specific sh-RNA of HOTTIP or negative. Note: sh-RNA (sh-con). RT-qPCR was used to detect HOTTIP level. (b) IR-K562 was transfected with specific sh-RNA of HOTTIP or negative sh-RNA (sh-con). CCK-8 analysis was used to detect cell proliferation. (c) CML cells were prepared as (a), and cell proliferation was detected by colony formation assay. vs. sh-con. (d) CML cells were prepared as (a), and cell apoptosis rate was detected by flow cytometry using Annexin V-APC/PI staining. The right panel shows the apoptosis rate from three independent experiments. , vs. sh-con. (e) Western blotting for protein levels in IR-K562 cells transfected with sh-HOTTIP and sh-con.

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