[Retracted] lncRNA HOTTIP Recruits EZH2 to Inhibit PTEN Expression and Participates in IM Resistance in Chronic Myeloid Leukemia

Figure 4

Knocking down HOTTIP increases the sensitivity of IR-K562 cells to IM. Note: (a) IR-K562 was transfected with specific sh-RNA of HOTTIP or negative sh-RNA (sh-con). RT-qPCR was used to detect HOTTIP level. CCK-8 analysis was used to detect cell proliferation. (b) IR-K562 cells were transfected with specific sh-HOTTIP or negative sh-con and treated with different Imatinib concentrations for 48 h. CCK-8 analysis was used to detect cell inhibition. vs. sh-con. (c) IR-K562 cells were transfected with specific sh-HOTTIP or negative sh-con and treated with different concentrations of Imatinib for 48 h. Cell apoptosis was detected by flow cytometry using Annexin V/APC/PI. The right panel shows the apoptosis rate from three independent experiments. vs.sh-on. (d) Western blotting for protein levels in IR-K562 cells transfected with sh-HOTTIP or negative sh-con and treated with IM (3 μM) or DMSO for 48 h.

(a)

(b)

(c)

(d)