[Retracted] lncRNA HOTTIP Recruits EZH2 to Inhibit PTEN Expression and Participates in IM Resistance in Chronic Myeloid Leukemia

Figure 7

The effect of HOTTIP on CML cell in vivo. Note: (a) Tazemetostat drug structure. (b) Analysis of H3K27me3. PTEN expression in IR-K562 cells treated by Tazemetostat with different concentrations for 48 h. vs. DMSO. (c) IR-K562 cells were engineered to stably knock down HOTTIP, and the cells were then subcutaneously injected into the nude mice to establish CML xenograft tumors. Tumor volumes were monitored by direct measurement. , , and vs.sh-con. Representative tumor sizes of xenograft mice in each group. The xenograft tumor wet weight in each group of mice. , vs. sh-con. (d) Immunohistochemistry stain was used to measure the PTEN, c-caspase3 protein levels in xenograft tumors. (e) Western blot was used to detect the H3K27me3, PTEN, and c-caspase3 protein levels in xenograft tumors.

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