Adipose-Derived Mesenchymal Stem Cell Facilitate Hematopoietic Stem Cell Proliferation via the Jagged-1/Notch-1/Hes Signaling Pathway

Figure 5

Effects of ADSCs on the proliferation of LSK cells in vitro after Jagged-1 knockdown. ADSCs were first subjected to Jagged-1 knockdown and then co-cultured with LSK cells. (a): Western blot analysis of Jagged-1 in ADSCs after Jagged-1 knockdown. (b): Real-time quantitative analysis of Jagged-1 in ADSCs after Jagged-1 knockdown. (c): The number of LSK cells at day 0 and after co-culture for 2 weeks and 5 weeks. (d): The cell morphology of coculture system. (e): Western blot analysis of Hes-1 in LSK cells. (f): Real-time quantitative analysis of Hes-1 in LSK cells. (g): Hematopoietic colony morphology after cultured with methylcellulose. .

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