Research Article
Metformin Ameliorates D-Galactose-Induced Senescent Human Bone Marrow-Derived Mesenchymal Stem Cells by Enhancing Autophagy
Figure 1
The cytotoxicity of D-gal on hBMSCs and effects of different concentrations D-gal on hBMSC senescence. (a) The cell viability of hBMSCs was assessed by CCK8 assay following treatment with different concentrations of D-gal (0-100 g/L) for 24 h. (b) Quantification of intracellular ROS levels by flow cytometry. (c) Representative images of flow cytometry of intracellular ROS levels. (d) Representative images of SA-β-Gal-positive hBMSCs, scale bar, 100 μm. (e) Quantification of MMP by flow cytometry. (f–h) The expressions of p21Waf1/Cip1 and p53 proteins were detected by western blot analysis. GAPDH was used as a control. for each group. Data shown are . Two-tailed test. , , , and versus the control group; ns: not significant.
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