Metformin Ameliorates D-Galactose-Induced Senescent Human Bone Marrow-Derived Mesenchymal Stem Cells by Enhancing Autophagy

Figure 2

The effects of metformin on improving senescence phenotypes of D-gal-induced hBMSCs. (a) The cell viability of hBMSCs was assessed by CCK8 assay after cultured for 72 h. (b) Quantification of intracellular ROS levels by flow cytometry. (c) Representative images of SA-β-Gal-positive hBMSCs, scale bar, 100 μm. (d) Quantification of MMP by flow cytometry. for each group. Data shown are . Two-tailed test. , , , and versus the D-gal group; ^## and ^#### versus the control group; ns: not significant.

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