Research Article
Metformin Ameliorates D-Galactose-Induced Senescent Human Bone Marrow-Derived Mesenchymal Stem Cells by Enhancing Autophagy
Figure 3
The effect of metformin on reversing cell cycle arrest in D-gal-induced hBMSCs. (a) Analysis of cell cycle distribution by flow cytometry. (b) Bar graph of cell cycle distribution. (c–f) The expressions of p16INK4a, p21Waf1/Cip1, and p53 proteins were detected by western blot analysis. GAPDH was used as a control. for each group. Data shown are . Two-tailed test. , , , and versus the D-gal group; ### and versus the control group; ns: not significant.
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