Donor Mesenchymal Stem Cells Program Bone Marrow, Altering Macrophages, and Suppressing Endometriosis in Mice

Figure 3

MSC program macrophage M1/M2 in vitro. (a) FACS analysis of macrophages cell line with cocultured with BM-derived MSCs. (i) M1 macrophages (iNOS⁺) were reduced by 30-fold in MSC cocultured cells compared to controls while (ii) M2 macrophages (CD163⁺) increased by fivefold. Experiments were performed three times each time in duplicate. (b) Western blot analysis of iNOS and CD163 showed protein levels of iNOS (i) were decreased while CD163 (ii) was increased in macrophages cocultured with MSCs from BM compared to macrophages cultured alone. The densitometry analysis of protein band ratios of iNOS and CD163 to β-actin are shown in bar graph (iii) and (iv), respectively. Protein levels of iNOS are significantly reduced while CD163 levels increased significantly in cocultured cells compared to macrophages alone. Each bar represents the mean ± SEM of two separate blots and each sample run in duplicate. between macrophages alone vs. coculture with MSCs.

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