Donor Mesenchymal Stem Cells Program Bone Marrow, Altering Macrophages, and Suppressing Endometriosis in Mice

Figure 4

Conditioning of BM with MSCs and cotransplantation did not result in MSC engraftment of BM however did program BM gene expression. (a) Flowcytometric analysis of BM cells after GFP-MSC cotransplantation demonstrated the complete absence of MSCs (CD45-CD34-GFP+) on Day 3 or Day 30 (n = 8). (b) Differential expression (mRNA levels) of CXCL12, CXCR4, IL6, and TNF-α in BMCs were analyzed by qRT-PCR at 30 days after BMT expressed relative to transcript level in BMCs of control mice not receiving transplant. Each bar represents the mean ± SEM of two individual experiments and each was performed in triplicates. BM vs. BM + MSCs (N = 8/group).

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