Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties

Table 1

Expression of markers for potential stem cells in different MSC sources.

Marker	MSCs source	% of cells	Comments	Reference
CD49f	BM	(1) 66.5; (2) 11	(1) Fetal BM; (2) Adult BM; -Loss CD49f expression during culture; -Differences between donors	[25]
CD49f	BM	22.2%	-Passage 1; -Loss during culture passages; -Differences between sources	[26]
CD49f	DM	6.68	-Passage 0; -Together with CD146 high expression	[27]
CD49f	(1) mouse AT; (2) rat AT	(1): 17.7; (2): 27.2	-Passage 2; -CD49f expression decreased after TNF and IFN treatment; -CD49f expression decreased with passage number	[28]
CD133	AT	12		[29]
CD133	BM	15		[29]
CD133	WJ	Less than 2		[29]
CD271	AFC	Less than 0.5		[30]
CD271	AT	8.4		[30]
CD271	AT	5		[29]
CD271	BM	(1) 22.3; (2) 10.1	(1) Young donors; (2) Elderly donors	[31]
CD271	BM	3.7		[30]
CD271	BM	8		[29]
CD271	BM	1.9	Organism: mouse	[32]
CD271	CB	Less than 0.5		[30]
CD271	DM	5.5		[33]
CD271	PVC	Less than 0.5		[30]
CD271	WJ	Less than 0.5		[30]
CD271	WJ	Less than 1		[34]
CD271	WJ	2		[29]
CD349	PL	20–58	-Site of isolation: chorion laeve tissue; -Expression differences between clones; -Loss of MSC markers; -Together with negative CD271 expression	[35]
CD349	PL	0.2	-Site of isolation not specified,; -Together with Nanog, Oct4 and SSEA4 upregulation	[36]
GD2	BM, adult	(1) ∼55; (2) ∼35	(1) Passage 2, cell culture in AB-HS; (2) Passage 2, cell culture in FBS	[37]
GD2	AT	46.7	–	[38]
GD2	BM	95	Passage 2, cells CD45^− CD105^+CD73^+, maintained expression for 8 passages	[39]
GD2	BM, fetal	(1) ∼88; (2) ∼65	(1) Passage 2, cell culture in AB-HS; (2) Passage 2, cell culture in FBS	[37]
GD2	BM	63.4–73.9	-Organism: mouse,; -Passage 2, expression differed between mouse strains	[40]
GD2	UC	(1) ∼38; (2) ∼18	(1) Passage 2, cell culture with AB-HS; (2) Passage 2, cell culture in FBS	[37]
Sca-1	BM	(1) 4; (2) 0.5	(1) Compact bone marrow; (2) Flushed bone marrow	[41]
SSEA-3	Mouse AT	6.3		[42]
SSEA-3	AT	3.2		[43]
SSEA-3	AT	8.8		[21]
SSEA-3	AT	1.9		[44]
SSEA-3	BM	5.3		[45]
SSEA-3	WJ (explant)	(1) 6.7; (2) 6.1	(1) Explant isolation, passage 0; (2) Enzymatic isolation, passage 0	[34]
SSEA-4	AT	10		[29]
SSEA-4	BM	(1) 5.2; (2) 4	(1) Young donors; (2) Elderly donors	[31]
SSEA-4	BM	(1) 72; (2) 79.8	(1) Females; (2) Males	[46]
SSEA-4	BM	55		[29]
SSEA-4	DM	5.6		[33]
SSEA-4	WJ	(1) 32.4; (2) 26.1	(1) Explant isolation, passage 0; (2) Enzymatic isolation, passage 0	[34]
SSEA-4	WJ	60		[29]
SSEA-4	WJ	(1) 35%; (2) 70%–74%	Used different human platelet lysates: (1) with lower concentration of factors, (2) with higher concentration of factors.	In submission

Note: ∼: approximately, AB-HS: human AB serum, AFC: amniotic fluid, AT: adipose tissue, BM: bone marrow, CB: cord blood, DM: dermis, FBS: fetal bovine serum, IFN: interferon, PL: placenta, PVC: perivascular compartment of umbilical cord, TNF: tumor necrosis factor, UC: umbilical cord, WJ: Wharton Jelly. Source of MSCs: human, unless otherwise stated.