Review Article

Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties

Table 4

Properties of subpopulations separated from MSC tissues.

MarkerSorting methodEffectReference

CD142FACS↑ Gene expression: SPARC, COL4A1, COL1A1, and COL5A1,
↑ “wound healing” potential,
↓ gene expression: MKI67,
↓ proliferation,
[109]

CD146↑ Proliferation,
↑ immunomodulation,
↑ multilineage differentiation,
[123]

CD146FACSNo differences in clonogenicity, proliferation, multilineage differentiation,
↑ differentiation in vascular smooth muscle cell
[124]

CD146FACS↑ Immunomodulation,
↑ telomere length
[125]

CD146FACS↑ Migration towards intervertebral discs,
↑ discogenic differentiation in vitro
[126]

CD146MACS↑ Adipogenesis,
↑ angiogenesis
[110]

CD146MACS↑ Differentiation,
↑ mineralization
[112]

CD200MoFlo (Dako, Glostrup, Denmark)↑ Level of αSM-actin protein,
↑ expression of RUNX2 and DLX5,
↑ osteoblastic differentiation
[114]

CD317FACSNo changes in clonogenicity,
smaller cells,
↑ increased IL-7 expression and secretion
[116]

CD317FACS↑ Expression of CD54, CXCL10, CXCL11 and CCL2,
induction of Th1 phenotype,
induction of cutaneous tissue damage of skin explant model of inflammation,
no tissue formation when applied in scaffold in immunocompromised mice
[117]

VCAMEasySep (magnetic separation)↑ Effective to modulate T helper subsets,
↓ clonogenecity
[120]

VCAMFACS↑ Angiogenic potential[121]

Note: FACS: fluorescence-activated cell sorting, IL: interleukin, MACS: magnetic-activated cell sorting.