Research Article

MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T Cells

Figure 3

The expressions of 7 mRNAs were identified in DC-CIK cells from AML patients. (a) The levels of the 7 mRNAs with the greatest expression differences were certified through applying the qRT-PCR assay in DC-CIK cells from normal and AML patients. After DC-CIK cells from AML patients were transfected with MMP9 or CCL1 shRNAs, the transfection effects were analyzed via qRT-PCR (b) and western blot assays (c). (d) After MMP9 or CCL1 knockdown, the concentration of LDH was confirmed by applying the LDH kit. , , and .
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